QF-PCR

QF-PCR (Quantitative Fluorescent Polymerase Chain Reaction) is a targeted molecular diagnostic test used for the rapid detection of the most common fetal chromosomal aneuploidies. It offers high accuracy in identifying trisomy 21 (Down syndrome), trisomy 18 (Edwards syndrome), trisomy 13 (Patau syndrome), and sex chromosome abnormalities such as Turner syndrome (45,X) and Klinefelter syndrome (47,XXY). The test can also detect triploidy and assess maternal cell contamination (MCC) in prenatal samples.
‍

By amplifying specific DNA markers across chromosomes of interest, QF-PCR delivers fast, reliable results without the need for cell culture—making it particularly useful when rapid decision-making is essential in prenatal care. It is commonly used as a first-line confirmatory test in pregnancies with abnormal screening results or ultrasound findings suggestive of chromosomal abnormalities.

‍

High-risk results from NIPT, double/triple marker, or ultrasound abnormalities
Pregnancies in women of advanced maternal age
Previous pregnancy affected by a chromosomal disorder
Early reassurance during invasive prenatal testing (CVS or amniocentesis)
Need for rapid aneuploidy diagnosis in time-sensitive clinical scenarios

Built for speed. Trusted by clinicians. Reassuring for families.

Trisomy 21 (Down syndrome)
Trisomy 18 (Edwards syndrome)
Trisomy 13 (Patau syndrome)
Sex chromosome aneuploidies:
Turner syndrome (45,X)
Klinefelter syndrome (47,XXY)
Triple X (47,XXX), XYY (47,XYY)
Triploidy (when applicable)
Maternal Cell Contamination (MCC) screening included

Prenatal Samples:
‍

Amniotic Fluid
- Collected via amniocentesis (typically between 15–20 weeks gestation)
- Volume: 15–20 ml
- Container: Sterile screw-capped container (no fixatives)
- Transport: Room temperature; deliver within 24 hours
- Important: Do not refrigerate, centrifuge, or freeze
  ‍

Chorionic Villi (CVS)
- Collected via chorionic villus sampling (typically between 11–14 weeks gestation)
- Amount: 10–20 mg of tissue
- Container: Sterile container with normal saline or transport medium
- Transport: Room temperature; deliver within 24 hours
- Important: Do not use preservatives or fixatives
  ‍

Pre-test genetic counseling is recommended for all invasive prenatal diagnostic procedures.

‍

Fast Turnaround:

Delivers results within 2–4 days—ideal for time-sensitive decisions in pregnancy
‍

High Sensitivity & Specificity:

Accurate detection of the most clinically relevant chromosomal aneuploidies
‍

Culture-Independent:

Not affected by cell viability, culture failure, or contamination
‍

Cost-Effective & Efficient:

Provides targeted information quickly with minimal sample requirement
‍

Supports Further Testing:

Can be followed by karyotype or microarray if broader analysis is needed

Built for speed. Trusted by clinicians. Reassuring for families.

QF-PCR at Labassure offers a fast, reliable, and focused solution for prenatal chromosomal analysis—empowering clinicians and expectant families with early, actionable insights in pregnancy management.

QF-PCR – Quantitative Fluorescent PCR

Built for speed. Trusted by clinicians. Reassuring for families.

Corporate

Test & Services

QF-PCR – Quantitative Fluorescent PCR

Built for speed. Trusted by clinicians. Reassuring for families.

QF PCR Aneuploidy Panel (13,18,21,X and Y)

QF PCR Aneuploidy Extended Panel (13,15,16,18,21,22,X and Y)

Corporate

Test & Services